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This study found that poly(ethylene-co-vinyl alcohol) membranes support the self-assembly of dermal papilla cells into compact spheroidal microtissues capable of inducing new hair follicles, suggesting potential for large-scale production for hair follicle regeneration.

This study found that 3D microtissue models of dermal papilla cells can enhance the ability to induce hair-follicle neogenesis in vivo, offering potential for controlled cell production in follicle regeneration.

This study developed a triple drug delivery system using nanoparticles loaded with 5-fluorouracil, curcumin, and piperine to target breast tumors, demonstrating notable in vitro cytotoxicity and anti-metastatic potential in 3D tumor spheroids which suggests its promise for further preclinical evaluation.

This study demonstrated that a new biomimetic tissue engineering strategy using DP spheroids with vascularization significantly restored the hair-inducing properties of dermal papilla cells compared to traditional 3D cultures.

In this study, fibronectin coating on a low-adhesive substratum increased cell aggregation, growth, and motility, enhancing the formation of hair follicle dermal papilla spheroids.

This study achieved a first by successfully forming large quantities of dermal papilla spheroidal microtissues, which retained their hair induction potential, using a micropatterned culture system.

This study found that forming spheroids using silicone micro-wells improved the viability and neural differentiation potential of human adipose-derived stem cells after thawing.

This study observed that using 3D dermal papilla microtissues enhances hair follicle regeneration and gene expression compared to 2D cultures, indicating their potential for hair growth drug screening.

This study found that a 3D Matrigel culture technique for dermal papilla cell spheroids can enhance hair follicle inductivity and induce hair-like fiber differentiation in vitro, even with high-passage cells.

This study found that both human and rat dermal papilla spheroids can induce hair follicle neogenesis, but larger spheroids increase inductivity without significantly affecting hair fiber diameter.

This study observed that a 3D culture model of hair follicle cells showed an anagen-like phase between days 3 to 6 and transitioned to a catagen-like phase by day 7, highlighting cell differentiation and structural development over time.

This study reports that using nanogel and layer-by-layer self-assembly to encapsulate single dermal papilla cells can form cell spheroids that regenerate hair follicles successfully in a hair follicle regeneration model.

This study reported that a novel chitosan/polyvinyl alcohol nanofiber sponge effectively enhances the hair follicle-inducing ability of dermal papilla multicellular spheroids in a mouse model.

In this study, researchers developed a microfluidics-based method for creating uniform, size-controlled stem cell spheroids that enhance osteogenic differentiation when encapsulated in alginate-RGD microgels.

This study investigated the use of 3D cell culture technology to explore hair follicle regeneration, examining the impact of the 3D cellular environment on hair follicle morphogenesis and the effects of microwell depth on spheroid formation.

This study demonstrated that using a perfusion culture bioreactor and 3D spheroid culture can enhance corneal endothelial cell expansion and support the construction of tissue-engineered corneal endothelial layers in vitro.

In this study, cell membrane remodeling with a thermoresponsive boronic acid copolymer was shown to rapidly form spheroids from cancer or cardiac cell lines under standard conditions, promising advances in tissue engineering.

This study developed a method for generating germ-like tissues called bbHFGs, which efficiently promoted hair follicle formation in mice, potentially advancing hair regenerative medicine applications.

This study introduces a spherical skin model that effectively mimics key features of human skin, offering a scalable and rapid alternative for non-animal dermatological and cosmetic testing.

This study found that using 3D dermal papilla spheroid models enhances extracellular matrix production and hair follicle marker expression, providing insights into hair follicle biology and potential for drug screening.

This study demonstrated that caffeine, minoxidil, and the HIF-1α-stimulating agent deferiprone significantly enhanced dermal papilla cell proliferation in a 3D culture model, suggesting deferiprone as a potential alternative to minoxidil.

The study found that growing dermal papilla cells in 3D spheroids enhances their activity with hair growth-promoting agents, such as minoxidil and TCQA, compared to traditional 2D cultures.

This review highlights recent advancements in microfluidics for 3D tissue model generation and discusses its applications in tissue engineering and high-throughput drug screening without reporting new experimental results.

This review highlighted that in an in vitro setting, 3D spheroid cultures of fish cells offer a promising alternative to traditional monolayer cultures, potentially advancing research in eco-toxicology, immunology, drug screening, and more by better mimicking in vivo conditions.

This review outlines the current state and future prospects of using layer-by-layer self-assembly for cell encapsulation in biomedical applications, such as cell-based biosensors, transplantation, and tissue engineering, while also discussing its limitations and potential advancements.

In this study, ultrasound was found to alter the distribution of fibronectin in collagen hydrogels, leading to enhanced microtissue formation, suggesting potential applications in tissue engineering.

This article discusses the concept of hair cloning through cultured follicular cell implantation but provides no new experimental results; it highlights ongoing interest among hair restoration professionals and the public.

This review discusses advances in using tissue engineering to improve the inductivity of dermal papilla cells for hair follicle restoration and reports no new clinical results.

This study found that host cells play a crucial role in the maturation of reconstructed hair follicles, emphasizing their participation in hair follicle reconstruction processes.