January 2010 in “Nihon Hou Kagaku Gijutsu Gakkaishi” This study concluded that their modified immunohistochemical staining protocol can determine the ABO blood group of both medullated and non-medullated scalp hair, potentially aiding in hair sample screening before DNA analysis.
January 1963 in “Stain technology” This study describes a staining technique that differentiates medullary from cortical keratin in sheep and goat hair using Ziehl-Neelsen's carbol-fuchsin in skin biopsy sections.
December 2022 in “Skin Pharmacology and Physiology” This study found that most hair shaft abnormalities can be accurately diagnosed using a handheld dermoscope in a clinical setting.
This study found that fluorescent antibody staining effectively differentiated between normal and tumor cells, with observed differences potentially linked to protein composition and developmental factors.
27 citations
,
February 2003 in “Cell and Tissue Research” This study found that FM dyes are effective markers for tracing live Merkel cells in in vitro experiments, as they remain visible for at least seven days and highlight cell shape better than quinacrine.
1 citations
,
January 2022 in “Anais brasileiros de dermatologia/Anais Brasileiros de Dermatologia” In this study, authors reported that reflectance confocal microscopy may aid in diagnosing trichofolliculoma by identifying specific features correlated with histopathology that can be difficult to discern with other methods.
13 citations
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August 2017 in “International Journal of Dermatology” This study reported that trichoscopy revealed distinctive patterns across different autoimmune bullous diseases, suggesting it can be a useful preliminary diagnostic tool for differentiating these conditions.
April 2021 in “Journal of Investigative Dermatology” A deep learning model was developed to help diagnose trichothiodystrophy by analyzing hair patterns.
December 2025 in “Y HOC TP HO CHI MINH” This study found that Trichoscope can effectively reveal clinical features of alopecia areata and androgenic alopecia, making it a useful tool for diagnosis and monitoring.
January 2026 in “Skin Appendage Disorders” This study concluded that including intermediate hairs or using relative shaft diameter methods can significantly improve the sensitivity of trichograms in diagnosing female androgenetic alopecia, aligning better with histopathology results.
23 citations
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November 2014 in “Anais Brasileiros de Dermatologia” This study suggests that trichoscopy may assist in diagnosing pemphigus, particularly for scalp-specific lesions, by identifying certain characteristic features.
This review discusses the use of hair microscopy in diagnosing hair-related conditions, highlighting techniques and common applications, but presents no new experimental results.
25 citations
,
November 2010 in “Journal of Molecular Structure” This preliminary study suggests that Raman micro-spectroscopy can help differentiate basal cell carcinoma from hair follicles in skin tissue sections, although some misclassification of hair follicles as carcinoma was observed.
This study found that dermoscopy combined with calcium fluorescent white staining effectively aided in the early diagnosis and treatment evaluation of tinea capitis by highlighting specific hair changes and fluorescent patterns associated with different dermatophyte infections in a sample of 20 patients.
7 citations
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June 2019 in “Coloration Technology” This research found that translucent keratin films absorbed more dye than opaque films, suggesting they may serve as better screening substrates for evaluating hair dye effectiveness.
November 2025 in “OPAL (Open@LaTrobe) (La Trobe University)” In this study, researchers conducted additional IHC staining on equine skin sections and found that collagen type III primarily labels the extracellular matrix, while anti-PDGFR-antibody staining highlights cells with long-shaped processes mainly arranged around hair follicles.
87 citations
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January 1999 in “British Journal of Dermatology” This study found that trichoblastic fibroma and basal cell carcinoma cannot be differentiated by cytokeratin expression patterns, while trichoepithelioma lacks CK7 expression, distinguishing it from the other two neoplasms.
4 citations
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December 2022 in “Skin Research and Technology” This study found that a combination of dermatoscopy and reflectance confocal microscopy assists in accurately diagnosing pigmented prurigo, which is often misdiagnosed due to its varied clinical manifestations.
29 citations
,
April 2011 in “Journal of Microscopy” This study describes a modified reduction-osmication method for staining human scalp hair that improves reagent penetration and visualization of hair cortex features using transmission electron microscopy.
1 citations
,
January 1995 in “Skin Cancer” This study suggests that immunohistochemistry of keratin and involucrin expression can aid in distinguishing malignant trichilemmoma from other tumors previously diagnosed as such.
16 citations
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June 1992 in “Journal of Investigative Dermatology”
28 citations
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August 2018 in “Dermatologic Clinics” This article discusses various aspects of trichoscopy for diagnosing hair and scalp disorders and reports no clinical results, offering guidance and highlighting pitfalls for better application of this tool by dermatologists.
21 citations
,
January 2000 in “Fetal Diagnosis and Therapy” This study found that trichothiodystrophy can be diagnosed prenatally in the second trimester using an endoscopically-guided fetal eyebrow biopsy, with sulfur content analysis being the most reliable test.
56 citations
,
January 1996 in “Journal of Forensic Sciences” This study found that rhodamine B penetrated human hair fibers more effectively from an aqueous solution compared to a methanol/ethanol solvent, with penetration depending on hair morphology.
19 citations
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July 1997 in “British Journal of Dermatology” This study successfully developed and characterized a monospecific monoclonal antibody, LHTric-1, that specifically localizes to the pre-cortical region of the hair follicle and can aid research on hair and nail formation.
9 citations
,
August 2017 in “Journal of comparative pathology” This study suggests that uncontrolled embryonic trichogenesis may play a role in the development of trichoblastoma in rabbits.
31 citations
,
February 2007 in “Journal of Structural Biology” Oxidation changes the structure of hair protein filaments, causing them to compact and rearrange.
3 citations
,
June 2023 in “Modern Pathology” This study found that GLI1 RNA CISH was highly sensitive and specific for distinguishing basal cell carcinoma from nonfollicular epithelial neoplasms but not from most benign follicular tumors.
2 citations
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October 2023 in “Dermatology practical & conceptual” This systematic review evaluated 60 studies on trichoscopy in hair and scalp disorders, revealing gaps in accounting for variables like disease stage or hair texture, and underscoring a need for more comprehensive analyses to fully understand trichoscopic patterns.
May 2022 in “Experimental dermatology” In this study, hair shafts from trichothiodystrophy patients with ERCC2 mutations revealed abnormal cuticle structures and protein imbalances compared to normal hair shafts.