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    Glossary Immunostaining

    technique using antibodies to detect specific proteins in tissues

    Immunofluorescence (IF) is a laboratory technique used to visualize the presence and location of specific proteins or antigens in cells or tissue sections by using antibodies that are chemically linked to a fluorescent dye. When these antibodies bind to their target, the fluorescent dye can be seen under a microscope with the appropriate light, allowing researchers to study the distribution and abundance of the proteins of interest.

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    1. Immunostaining study of cytokeratins in human hair follicle development 2020 · 2 citations
    2. New Insight Into the Immunostaining Method Suitable for the Pilosebaceous Complex Unit 2015
    3. Human Dermal Fibroblasts Demonstrate Positive Immunostaining for Neuron- and Glia- Specific Proteins PLoS ONE · 2015 · 15 citations
    4. Investigating the role of various extracellular matrix in androgenetic alopecia: Insights from immunostaining and quantitative analysis – A pilot study Dermatologica Sinica · 2024
    5. Intradermal adipocytes mediate fibroblast recruitment during skin wound healing Development · 2013 · 238 citations
    6. Cytokeratin expression in trichoblastic fibroma (small nodular type trichoblastoma), trichoepithelioma and basal cell carcinoma British Journal of Dermatology · 1999 · 87 citations
    7. Melanocyte Subpopulation Turnover During the Human Hair Cycle: An Immunohistochemical Study Pigment cell research · 2000 · 86 citations
    8. YAP and TAZ are essential for basal and squamous cell carcinoma initiation EMBO Reports · 2018 · 76 citations
    9. Targeted Expression of Human Vitamin D Receptor in the Skin Promotes the Initiation of the Postnatal Hair Follicle Cycle and Rescues the Alopecia in Vitamin D Receptor Null Mice 2002 · 57 citations
    10. Effects of Finasteride on Apoptosis and Regulation of the Human Hair Cycle Journal of Cutaneous Medicine and Surgery · 2002 · 21 citations
    11. Characterization of LHTric-1, a new monospecific monoclonal antibody to the trichocyte keratin Ha1 British Journal of Dermatology · 1997 · 19 citations
    12. Alterations in Hair Follicle Morphology and Hair Shaft Production After Follicular Unit Transplantation The American Journal of Dermatopathology · 2016 · 8 citations
    13. Research Techniques Made Simple: Optical Clearing and Three-Dimensional Volumetric Imaging of Skin Biopsies Journal of Investigative Dermatology · 2020 · 2 citations
    14. Lentiginous melanoma. A clinically malignant entity that histopathologically seems benign. Case study harbouring BRAF V 600R mutation Journal of the European Academy of Dermatology and Venereology · 2015 · 1 citations
    15. 854 Dermal fibroblasts enhance growth factors production by androgens, and modulate keratinocyte proliferation and differentiation 2018
    16. 1337 Single cell transcriptome profiling of human interfollicular epidermis reveals robust heterogeneity and divergent differentiation lineages Journal of Investigative Dermatology · 2018
    17. 883 Hair follicle bulb developed as 3D scaffold free microtissue Journal of Investigative Dermatology · 2017
    18. Drug delivery to the hair follicle : role of follicular tight junctions as a biological barrier and the potential for targeting clobetasol nanocarriers SciDok (Saarland University and State Library) · 2015
    19. Reconstitution of human skin in vitro using readily available sources: Novel studies of tumour promotion Toxicology in Vitro · 1994
    20. Follicular Stem Cells in Androgenetic Alopecia Journal of Cytology and Histology · 2015
    21. Immunofluorescence Tomography: High-resolution 3-D reconstruction by serial-sectioning of methacrylate embedded tissues and alignment of 2-D immunofluorescence images Scientific Reports · 2019 · 8 citations
    22. Expression of the hair stem cell-specific keratin 15 in pilar tumors of the skin. PubMed · 1999 · 54 citations
    23. Reply to ‘CD123 immunohistochemistry for plasmacytoid dendritic cells is useful in the diagnosis of scarring alopecia’: three PDC-related parameters are useful in differentiating lupus alopecia from LPP Journal of Cutaneous Pathology · 2016 · 4 citations
    24. Condylomata acuminata of HIV-positive men may harbour focal areas of dysplasia: relevant implications for the management of human papillomavirus-induced disease in high-risk patients British Journal of Dermatology · 2016 · 3 citations
    25. Cicatricial alopecia of the scalp as a manifestation of IgG4-related disease mimicking acne keloidalis nuchae Journal of Cutaneous Immunology and Allergy · 2026
    26. Squaric Acid Dibutylester Promotes Innate Immune-Driven Hair Growth with CD206+ Macrophage Accumulation in the Dermis JID Innovations · 2025
    27. l-Ascorbic acid 2-phosphate promotes elongation of hair shafts via the secretion of insulin-like growth factor-1 from dermal papilla cells through phosphatidylinositol 3-kinase 2009 · 34 citations
    28. Trichodysplasia spinulosa: a polyomavirus infection specifically targeting follicular keratinocytes in immunocompromised patients 2016 · 19 citations
    29. Clinical, Trichoscopic And Immunohistochemical Evaluation of Autologous Adipose Derived Adult Stem Cell Injection in the Treatment of Female Pattern Hair Loss QJM: An International Journal of Medicine · 2021
    30. Fluorescence Activated Cell Sorting (FACS) of CK15 Cells in Canine Hair Follicular Tissue International Journal of Current Microbiology and Applied Sciences · 2020