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This article details laboratory methods for isolating and culturing human dermal papilla cells but provides no new experimental results.

This review discusses current experimental approaches for regenerating human hair follicles using tissue engineering and isolated cells, but reports no successful strategies with adult cells yet found.

The study suggests that culturing adipose-derived mesenchymal stem cells into spheroids with Wnt exposure can result in cell clusters resembling hair follicle dermal papilla cells, potentially aiding hair follicle generation.

This study developed and validated a sensitive method using adsorptive cathodic stripping voltammetry to directly determine minoxidil in topical solutions, shampoos, and creams, achieving high recovery rates and precision.

Growing hair follicles from cultured cells could potentially treat baldness, but more research is needed.

This study describes a protocol for generating hair follicle germ-like organoids from human skin cells, which may aid in alopecia drug discovery and understanding hair follicle regeneration.

This study found that while extracellular matrix components like hyaluronic acid promoted larger organoid formation, other matrix components hindered hair follicle germ assembly in an in vitro model.

This study observed that a 3D culture model of hair follicle cells showed an anagen-like phase between days 3 to 6 and transitioned to a catagen-like phase by day 7, highlighting cell differentiation and structural development over time.

This study found that biomimetic dermal papilla spheres cultured in a specific microenvironment can partially restore hair-inducing ability in high-passage dermal papilla cells in nude mice, resembling the characteristics of primary cells.

This study found that 3D microtissue models of dermal papilla cells can enhance the ability to induce hair-follicle neogenesis in vivo, offering potential for controlled cell production in follicle regeneration.

This study found that hyaluronic acid increased the size of hair follicle germ-like aggregates and the number of proliferative cells but did not maintain specific markers during the process.

In this study, researchers differentiated human induced pluripotent stem cells into dermal papilla-like cells and observed gene expression dynamics during five stages of dermal differentiation, demonstrating the potential of these cells to interact with epidermal cells in functional assays.

This study developed an in vitro artificial hair germ model using dermal papilla cells and skin keratinocytes to explore early stages of hair follicle regeneration.

This study found that anisotropic osmolyte solutions may improve hair follicle turnover and condition in both in vitro and in silico models, as well as in human volunteers with hair disorders.

This review discusses recent advances in developmental tissue engineering for regenerating ectodermal appendages like teeth and glands, emphasizing biomaterial selection and cell culture strategies, but reports no new experimental results.

This review discusses recent advances in microfabrication and microfluidic technologies for improving the production of organoids and spheroids, but it reports no new experimental results.

In this study of patients with Androgenic Alopecia, micro-grafts containing HD-AFSCs resulted in significantly improved hair density and follicle count over 11 months compared to a saline solution, suggesting a potential alternative therapy for hair regrowth.

In this study, the researchers reported that their proposed methods accurately determined the studied drug with good recovery rates despite the presence of oxidative degradation products.

This study found that their novel 3D-imaging method, ASAXA-μCT, identified shrinkage of sweat glands as a key factor in skin aging, which leads to dermal defects, decreased elasticity, and increased wrinkling and sagging.

This review discusses the development and advances in studying trichogenic dermal cells for hair follicle morphogenesis, summarizing methods, bioassays, and molecular markers, but reports no new research findings.

This study found that the human placenta extracellular matrix hydrogel restored the hair-inductive capacity of high-passaged dermal papilla cells, enabling them to regenerate new hair follicles when co-grafted with mouse epidermal cells.

This review discusses hair follicle organoids as emerging models for studying hair biology and disorders, emphasizing their promise for bridging basic research and clinical applications, but reports no new results.

This study reviewed various methods and techniques aimed at overcoming limitations in culturing human hair follicles, specifically focusing on restoring the inductivity of hair follicle cells for successful hair regeneration.

Innovative methods are reducing animal testing and improving biomedical research.

This article reviews methods to enhance epithelial–mesenchymal interactions for hair follicle bioengineering but does not present new experimental results; it emphasizes optimizing combinations for successful regeneration.

Method measures minoxidil in tablets accurately and easily.

In this study, researchers developed a microfluidics-based method for creating uniform, size-controlled stem cell spheroids that enhance osteogenic differentiation when encapsulated in alginate-RGD microgels.

This study developed a method for generating germ-like tissues called bbHFGs, which efficiently promoted hair follicle formation in mice, potentially advancing hair regenerative medicine applications.

This study demonstrated a method for large-scale preparation of self-sorted hair follicle germs in vitro, which successfully generated hair follicles when transplanted into the skin of nude mice.

This study developed a new analytical method to identify a wide range of endocrine disrupting compounds in full-term amniotic fluid, revealing diverse substances with potential endocrine activity.