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    Glossary Hanging Drop Culture

    suspends cells in a liquid drop for microscopic observation

    The Hanging Drop Culture is a laboratory technique used to study living cells, such as bacteria or hair follicle cells, in a drop of liquid suspended from a coverslip. This method allows for the observation of cell behavior and interactions in a more natural, three-dimensional environment compared to traditional flat cultures.

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    Research 30 of 311

    1. Cultured follicles offer hope for beating baldness Nature · 2013
    2. Methods for the isolation and 3D culture of dermal papilla cells from human hair follicles Experimental Dermatology · 2017 · 55 citations
    3. Hair Germ Model In Vitro via Human Postnatal Keratinocyte-Dermal Papilla Interactions: Impact of Hyaluronic Acid Stem cells international · 2017 · 42 citations
    4. Generation of Hair Follicle Germs In Vitro Using Human Postnatal Skin Cells Methods in molecular biology · 2020 · 2 citations
    5. Morphogenetic Events in Hair Follicle Heterotypic Spheroids 2017
    6. Trajectory of hiPSCs derived neural progenitor cells differentiation into dermal papilla-like cells and their characteristics Scientific reports · 2023
    7. Hair follicle regeneration in vitro Paleontological Journal · 2016
    8. Epidermal stem cells and skin tissue engineering in hair follicle regeneration World Journal of Stem Cells · 2015 · 83 citations
    9. Surface Tension Guided Hanging-Drop: Producing Controllable 3D Spheroid of High-Passaged Human Dermal Papilla Cells and Forming Inductive Microtissues for Hair-Follicle Regeneration ACS Applied Materials & Interfaces · 2016 · 62 citations
    10. Generating hair follicle inductive dermal papillae cells from adipose derived mesenchymal stem cells Belarusian State Pedagogical University repository (Belarusian State Pedagogical University) · 2018
    11. 883 Hair follicle bulb developed as 3D scaffold free microtissue Journal of Investigative Dermatology · 2017
    12. Hair Strengthening Evaluation of Anisotropic Osmolite Solutions (Inositol + Arginine): Cross-Talk between Dermal Papilla Fibroblast and Keratinocytes of the Outer Root Sheath Using a µHair Follicle 3D Model Cosmetics · 2018
    13. Biological activity and hair inducing function of biomimetic dermal papilla spheres prepared by hanging drops of methacrylate gelatin 2021 · 1 citations
    14. Scaffold-based developmental tissue engineering strategies for ectodermal organ regeneration Materials Today Bio · 2021 · 22 citations
    15. Enhanced viability and neural differential potential in poor post-thaw hADSCs by agarose multi-well dishes and spheroid culture Human Cell · 2015 · 15 citations
    16. RNA sequence analysis of dermal papilla cells’ regeneration in 3D culture Journal of Cellular and Molecular Medicine · 2020 · 6 citations
    17. HIF-1α Stimulators Function Equally to Leading Hair Loss Agents in Enhancing Dermal Papilla Growth Skin Pharmacology and Physiology · 2020 · 6 citations
    18. Hair Follicle Reconstruction and Stem Cells InTech eBooks · 2017 · 2 citations
    19. Use of extracellular matrix hydrogel from human placenta to restore hair-inductive potential of dermal papilla cells Regenerative Medicine · 2019 · 31 citations
    20. Hair follicle organoids: advances in construction, applications, and translational challenges Frontiers in Cell and Developmental Biology · 2026
    21. Faculty Opinions recommendation of Microenvironmental reprogramming by three-dimensional culture enables dermal papilla cells to induce de novo human hair-follicle growth. Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · 2013
    22. Culture and Differentiation of Human Hair Follicle Dermal Papilla Cells in a Soft 3D Self-Assembling Peptide Scaffold Biomolecules · 2020 · 29 citations
    23. Comparative analysis of 3D and 2D cell-culturing methods in hair follicle spheroid morphogenesis and drug responsiveness Biomaterials Advances · 2025 · 1 citations
    24. Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering Nanomaterials · 2024 · 1 citations
    25. Transcriptome Analysis Reveals an Inhibitory Effect of Dihydrotestosterone-Treated 2D- and 3D-Cultured Dermal Papilla Cells on Hair Follicle Growth Frontiers in Cell and Developmental Biology · 2021 · 5 citations
    26. Hair Follicle Dermal Cells Support Expansion of Murine and Human Embryonic and Induced Pluripotent Stem Cells and Promote Haematopoiesis in Mouse Cultures Stem cells international · 2018 · 3 citations
    27. Differentiation of Human Pluripotent Stem Cells Into Definitive Endoderm Cells in Various Flexible Three-Dimensional Cell Culture Systems: Possibilities and Limitations Frontiers in Cell and Developmental Biology · 2021 · 13 citations
    28. Establishment of an immortalized mouse dermal papilla cell strain with optimized culture strategy PeerJ · 2018 · 3 citations
    29. Disease-related biomarkers as experimental endpoints in 3D skin culture models Cytotechnology · 2023 · 3 citations
    30. Hair morphogenesis<i>in vitro</i>: formation of hair structures suitable for implantation Regenerative Medicine · 2008 · 43 citations