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    Glossary Hanging Drop Culture

    suspends cells in a liquid drop for microscopic observation

    The Hanging Drop Culture is a laboratory technique used to study living cells, such as bacteria or hair follicle cells, in a drop of liquid suspended from a coverslip. This method allows for the observation of cell behavior and interactions in a more natural, three-dimensional environment compared to traditional flat cultures.

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    Research 30 of 308

    1. Generation of Hair Follicle Germs In Vitro Using Human Postnatal Skin Cells Methods in molecular biology · 2020 · 2 citations
    2. Methods for the isolation and 3D culture of dermal papilla cells from human hair follicles Experimental Dermatology · 2017 · 55 citations
    3. Epidermal stem cells and skin tissue engineering in hair follicle regeneration World Journal of Stem Cells · 2015 · 83 citations
    4. Trajectory of hiPSCs derived neural progenitor cells differentiation into dermal papilla-like cells and their characteristics Scientific reports · 2023
    5. Hair Germ Model In Vitro via Human Postnatal Keratinocyte-Dermal Papilla Interactions: Impact of Hyaluronic Acid Stem cells international · 2017 · 42 citations
    6. 883 Hair follicle bulb developed as 3D scaffold free microtissue Journal of Investigative Dermatology · 2017
    7. Scaffold-based developmental tissue engineering strategies for ectodermal organ regeneration Materials Today Bio · 2021 · 22 citations
    8. Hair Strengthening Evaluation of Anisotropic Osmolite Solutions (Inositol + Arginine): Cross-Talk between Dermal Papilla Fibroblast and Keratinocytes of the Outer Root Sheath Using a µHair Follicle 3D Model Cosmetics · 2018
    9. Hair follicle organoids: advances in construction, applications, and translational challenges Frontiers in Cell and Developmental Biology · 2026
    10. Differentiation of Human Pluripotent Stem Cells Into Definitive Endoderm Cells in Various Flexible Three-Dimensional Cell Culture Systems: Possibilities and Limitations Frontiers in Cell and Developmental Biology · 2021 · 13 citations
    11. Culture and Differentiation of Human Hair Follicle Dermal Papilla Cells in a Soft 3D Self-Assembling Peptide Scaffold Biomolecules · 2020 · 29 citations
    12. Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering Nanomaterials · 2024 · 1 citations
    13. Large-scale cultivation of transplantable dermal papilla cellular aggregates using microfabricated PDMS arrays Acta Biomaterialia · 2010 · 25 citations
    14. Sustained release of dermal papilla-derived extracellular vesicles from injectable microgel promotes hair growth Theranostics · 2020 · 46 citations
    15. 3D Spheroid Human Dermal Papilla Cell as an Effective Model for the Screening of Hair Growth Promoting Compounds: Examples of Minoxidil and 3,4,5-Tri-O-Caffeoylquinic Acid (TCQA) Cells · 2022 · 2 citations
    16. Mimicking Senescence Factors to Characterize the Mechanisms Responsible for Hair Regression and Hair Loss: An In Vitro Study Organoids · 2025
    17. Microfluidic Technology for the Generation of Cell Spheroids and Their Applications Micromachines · 2017 · 125 citations
    18. Engineering immune-competent hair follicle microphysiological systems: from organoid assembly to dynamic immune modelling Frontiers in Bioengineering and Biotechnology · 2026
    19. RNA sequence analysis of dermal papilla cells’ regeneration in 3D culture Journal of Cellular and Molecular Medicine · 2020 · 6 citations
    20. Enhanced viability and neural differential potential in poor post-thaw hADSCs by agarose multi-well dishes and spheroid culture Human Cell · 2015 · 15 citations
    21. Systematic Review of Platelet-Rich Plasma Use in Androgenetic Alopecia Compared with Minoxidil®, Finasteride®, and Adult Stem Cell-Based Therapy International Journal of Molecular Sciences · 2020 · 65 citations
    22. HIF-1α Stimulators Function Equally to Leading Hair Loss Agents in Enhancing Dermal Papilla Growth Skin Pharmacology and Physiology · 2020 · 6 citations
    23. Use of extracellular matrix hydrogel from human placenta to restore hair-inductive potential of dermal papilla cells Regenerative Medicine · 2019 · 31 citations
    24. Hair Follicle Reconstruction and Stem Cells InTech eBooks · 2017 · 2 citations
    25. From basic biology to engineered therapies: the keratinocyte stem cell playbook Frontiers in Medical Technology · 2026
    26. 879 Molecular network of Smads and Id2 genes in hair follicle stem cells regulation Journal of Investigative Dermatology · 2017
    27. Organogenesis From Dissociated Cells: Generation of Mature Cycling Hair Follicles From Skin-Derived Cells Journal of Investigative Dermatology · 2005 · 205 citations
    28. Hair morphogenesis<i>in vitro</i>: formation of hair structures suitable for implantation Regenerative Medicine · 2008 · 43 citations
    29. Faculty Opinions recommendation of Microenvironmental reprogramming by three-dimensional culture enables dermal papilla cells to induce de novo human hair-follicle growth. Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · 2013
    30. Hair Follicle Dermal Cells Support Expansion of Murine and Human Embryonic and Induced Pluripotent Stem Cells and Promote Haematopoiesis in Mouse Cultures Stem cells international · 2018 · 3 citations