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This study developed an in vitro model revealing that hair follicle spheroids enhanced both sweat gland and hair follicle differentiation within bioprinted scaffolds, while microenvironmental cues supported persistent regeneration.

This study established a new in vitro culture system that supports the growth and dynamic self-organization of hair follicle stem cells and their progeny, promoting a stable population equilibrium.

This study suggests that exosomes derived from dermal papilla cells, especially those cultured in three dimensions, promote hair growth and regeneration by enhancing follicular cell activity.

This study demonstrated that hair follicle-derived cells can induce hair follicle formation in an in vitro skin organ culture system using human retroauricular skin specimens.

This article details laboratory methods for isolating and culturing human dermal papilla cells but provides no new experimental results.

This study found that human adipose-derived stem cells co-cultured on a collagen sponge scaffold showed increased differentiation into keratinocytes, suggesting potential for improved skin wound healing.

Cosmetics enhance beauty, fix defects, and intimidate enemies, with varying cultural standards and alternative methods.

This article reviews organotypic skin culture systems and their applications in dermatological research, highlighting their ability to model human epidermal conditions in vitro, but it reports no new clinical results.

This study reported the successful isolation of epithelial stem cells from mouse embryonic skin using a simple culture method, with the cells showing the potential to differentiate into multiple cell types.

This study found that dermal papilla cells in culture may enhance and attract follicular epithelial cell growth, suggesting their pivotal role in hair and follicle development.

The new device improves human hair follicle cell growth and differentiation.

This study found that conditioned medium from keratinocytes improved the hair-inductive properties of cultured dermal papilla cells, suggesting potential for enhancing hair follicle regeneration.

This study found that silibinin may promote hair growth by increasing proliferation and activating the AKT and Wnt/β-catenin signaling pathways in 3D cultured human dermal papilla spheroids.

In this study, minoxidil sulphate was observed to significantly increase DNA synthesis in cultured human hair follicles in a concentration-dependent manner.

This study found that dermal papilla cells cultured in vitro can promote hair follicle regeneration and maintain hair growth, with stronger ET-1 and SCF expression enhancing this regenerative ability.

Skin organ culture helps us understand skin biology and diseases better.

This review discusses various approaches to treating alopecia, including drug therapy and hair transplantation, but reports no new research findings; the authors highlight the need for novel techniques like cell culturing.

This study found that a novel 3D in vitro culture condition improved stemness markers and proliferation potential in human dermal stem/progenitor cells compared to traditional 2D methods.

This study concluded that the new subfractionation culturing method generates highly homogeneous adipose-derived stem cells with enhanced mitogenic, paracrine, and hair growth-promoting effects compared to traditional isolation methods.

This study found that SFRP2 enhances Wnt3a-mediated β-catenin signaling in human dermal papilla cells, with higher SFRP2 expression in beard cells correlating with increased trichogenicity.

This study established an efficient short-term culture system for human hair follicle stem cells using human fibronectin and ROCK inhibitor Y-27632, enhancing cell proliferation and stemness for potential applications in hair follicle tissue engineering.

This study found that forming spheroids using silicone micro-wells improved the viability and neural differentiation potential of human adipose-derived stem cells after thawing.

This study demonstrated that using a perfusion culture bioreactor and 3D spheroid culture can enhance corneal endothelial cell expansion and support the construction of tissue-engineered corneal endothelial layers in vitro.

This narrative review explores how exosomes and cell culture-conditioned media are becoming key players in the cosmetic industry for skin rejuvenation and prejuvenation, emphasizing their multifunctional topical benefits and potential future applications.

This study describes a method for serially cultivating adult human hair follicle keratinocytes using a specific growth substrate and cryogenic storage, enabling subculture and immunofluorescence studies.

This study found that a titrated extract of Centella asiatica enhanced the hair growth potential of 3D spheroid cultured human dermal papilla cells.

In this study, an immortalized MSC line from human adipose tissue showed potential for applications in regenerative medicine and inflammatory diseases through production of active factors, demonstrated in vitro.

This study reported that a novel cell culture medium gel significantly increased hair counts and reduced hair loss in men with androgenetic alopecia over a six-month period.

In this study, cyclosporine A stimulated hair growth in mouse vibrissae organ culture by promoting matrix cell proliferation and altering growth factor expression.

This article discusses the factors contributing to acne and reports no new findings, focusing on the role of sebaceous lipids and associated processes.