Search

everythingexperts & clinicslearnfaqsresearchcommunity

for

Search:↓

This study investigated how 2D and 3D cell culturing methods influence hair follicle morphogenesis, finding that 3D cultures responded more expectedly to minoxidil while 2D cultures reacted better to DHT treatment, challenging the assumption that 3D cultures are always superior.

This review discusses advances in using tissue engineering to improve the inductivity of dermal papilla cells for hair follicle restoration and reports no new clinical results.

This article details laboratory methods for isolating and culturing human dermal papilla cells but provides no new experimental results.

This review discusses various approaches to treating alopecia, including drug therapy and hair transplantation, but reports no new research findings; the authors highlight the need for novel techniques like cell culturing.

This study reviewed various methods and techniques aimed at overcoming limitations in culturing human hair follicles, specifically focusing on restoring the inductivity of hair follicle cells for successful hair regeneration.

This study found that a novel 3D in vitro culture condition improved stemness markers and proliferation potential in human dermal stem/progenitor cells compared to traditional 2D methods.

This study investigated the use of 3D cell culture technology to explore hair follicle regeneration, examining the impact of the 3D cellular environment on hair follicle morphogenesis and the effects of microwell depth on spheroid formation.

This review discusses current experimental approaches for regenerating human hair follicles using tissue engineering and isolated cells, but reports no successful strategies with adult cells yet found.

Early attempts at using cloned cells for hair transplants failed, but 3D cell growth showed some promise.

This review discusses recent advancements in the study and application of epidermal stem cells for wound healing and tissue engineering, without presenting new experimental findings.

Three-dimensional culture helps dermal papilla cells grow new human hair follicles.

This study observed that a 3D culture model of hair follicle cells showed an anagen-like phase between days 3 to 6 and transitioned to a catagen-like phase by day 7, highlighting cell differentiation and structural development over time.

This study found that 3D spheroid culture reprogrammed mesenchymal stem cells into a uniform immunosuppressive phenotype, suggesting potential therapeutic applications for inflammatory diseases like psoriasis.

This study demonstrated that culturing high-passage dermal papilla cells with specific supplemented conditioned media may preserve their hair-inducing abilities, suggesting potential for reconstructing new hair follicles in vivo.

This study found that mammary resident macrophages regulate mammary epithelium cell division and development, with implications for maintaining mammary stem cell activity and homeostasis.

This study developed a biomimetic 3-D co-culture system that enhances the hair-inductive properties of human dermal papilla cell aggregates, potentially improving hair follicle tissue engineering.

This study introduces a magnet-assisted fabrication strategy for creating complex 3D soft bioscaffolds, demonstrating its effectiveness by producing structures with overhangs and supporting biohybrid actuators, promising advancements in biomedical applications.

This study found that mammary resident macrophages play a key role in mammary tissue development by influencing cell division and maintaining mammary stem cell activity through the TNF-α-Cdk1/Cyclin B1 signaling pathway, highlighting their importance in the mammary stem cell niche.

At a workshop highlighted during the 2025 ESPCR meeting, researchers discussed the challenges and variability in culturing skin-related cells, identifying key factors such as media composition and species differences that affect experimental reproducibility, and emphasized the importance of transparent practices to advance pigment cell research.

This review discusses the development and application of cell-derived matrices in regenerative medicine and disease modeling, presenting various fabrication techniques and future perspectives, but reports no new results.

This review discusses how recent advances in live imaging have revealed the dynamic and adaptable nature of stem cells during various biological processes, and reports no clinical results.

This study developed thermo-reversible glycol chitosan hydrogels that effectively form and maintain 3D cell spheroids within one day, offering a simplified method for creating biologically realistic cultures for tissue regeneration and drug screening applications.

This study found that a 3D co-culture of endothelial colony-forming cells and adipose-derived stem cells restored stem cell properties and improved healing in a mouse model of chronic injury.

The study found that growing dermal papilla cells in 3D spheroids enhances their activity with hair growth-promoting agents, such as minoxidil and TCQA, compared to traditional 2D cultures.

This review found that 3D bioprinting significantly advances skin tissue engineering by enabling the creation of complex, patient-specific skin structures, though technological and regulatory challenges persist, particularly in areas like scalability and physiological mimicry.

This study demonstrated that a 3D air–liquid culture system using Wnt-CM-treated cells can induce hair regeneration in nude mice and may facilitate large-scale preparation for hair loss treatment.

This study demonstrated that PEGDA hydrogel microwells successfully supported cell survival and proliferation while mimicking hair follicle architecture in vitro, suggesting a potential tool for hair follicle engineering.

This study found that tdDPC-EVs significantly improved wound healing by enhancing angiogenesis through the KLF4/VEGFA axis, offering advantages over traditional DPC-EVs.

This study demonstrated that using a perfusion culture bioreactor and 3D spheroid culture can enhance corneal endothelial cell expansion and support the construction of tissue-engineered corneal endothelial layers in vitro.

This review discusses current knowledge of keratinocyte stem cell dynamics, including their regenerative roles and potential applications beyond wound healing, but presents no new clinical findings.