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This study investigated how 2D and 3D cell culturing methods influence hair follicle morphogenesis, finding that 3D cultures responded more expectedly to minoxidil while 2D cultures reacted better to DHT treatment, challenging the assumption that 3D cultures are always superior.

This study observed that dermal papilla cell aggregates showed increased viability and gene expression on softer 3D substrates, indicating the importance of substrate stiffness in cellular behavior.

This study demonstrated that PEGDA hydrogel microwells successfully supported cell survival and proliferation while mimicking hair follicle architecture in vitro, suggesting a potential tool for hair follicle engineering.

This study found that 3D cultured hair follicle-like constructs, which include keratinocytes and dermal papilla cells, showed improved gene expression and cell interactions relevant to hair follicle development.

This review discusses recent advances in surface modification and endothelialization of biomaterials for vascular grafts, highlighting promising methods like gene engineering and targeting ligand immobilization to improve clinical outcomes.