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research Long-term expansion and differentiation of adult murine epidermal stem cells in 3D organoid cultures

67 citations , June 2019 in “Proceedings of the National Academy of Sciences”

This study introduces a method for long-term expansion of adult mouse epidermal stem cells in vitro using an organoid culture system that maintains the basal-apical organization.

research Impaired differentiation potential of CD34-positive cells derived from mouse hair follicles after long-term culture

2 citations , June 2022 in “Scientific reports”

In this study, one type of commercial culture medium supported expression of stem cell markers in hair follicle epithelial stem cells, but their differentiation potential and hair follicle-inducing ability declined over time.

research Long‐term culture of skin biopsies: maintenance of fibroblast production and competency of reprogramming

December 2025 in “FEBS Open Bio”

In this study, fibroblasts from long-term skin biopsy cultures retained their ability to reprogram into induced pluripotent stem cells after 16 months, despite undergoing transcriptional changes and decreased proliferation rates over successive generations.

A Model System for Long-Term Serum-Free Suspension Organ Culture of Human Fetal Tissues: Experiments on Digits and Skin from Multiple Body Regions

research A model system for long-term serum-free suspension organ culture of human fetal tissues: experiments on digits and skin from multiple body regions

17 citations , January 1997 in “Cell and Tissue Research”

This study found that a serum-free DMEM-F12 medium with 45% oxygen best supports in vitro development and differentiation of human fetal skin, closely mimicking the in vivo state.

research Long-term follow-up of leucoderma patients treated with transplants of autologous cultured melanocytes, ultrathin epidermal sheets and basal cell layer suspension

177 citations , November 2002 in “British journal of dermatology/British journal of dermatology, Supplement”

In this study, stable types of leucoderma such as segmental vitiligo and piebaldism showed 100% repigmentation after melanocyte transplantation, suggesting surgery as the preferred method for these conditions.

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community Has anyone here been on finasteride for longer than 10 years? If so, have you had any major issues with the medicine please share your experience and how long you have taken the medicine.

in Treatment 112 upvotes 9 months ago

Long-term finasteride use typically slows hair loss, with some users experiencing side effects like sexual dysfunction and depression. Many users also use minoxidil and dutasteride for improved results, though individual responses vary.

community Has anyone here actually tried Shiseido’s S-DSC hair regenerative treatment in Japan?

14 upvotes 4 months ago

Shiseido's S-DSC hair regenerative treatment in Japan focuses on improving existing hair by thickening miniaturized follicles, not creating new ones. There is limited patient feedback and long-term data available.

community We need more people trying 2.5 mg dutasteride

in Finasteride/Dutasteride 7 upvotes 1 year ago

Increasing the dutasteride dose to 2.5 mg may significantly reduce DHT and promote hair regrowth, with similar side effects to lower doses. Some users combine dutasteride with minoxidil and finasteride for better results, but there are concerns about long-term effectiveness and availability.

community Minoxidil works amazingly, you just need to give it time.

326 upvotes 9 months ago

Minoxidil can effectively promote hair regrowth with consistent use, but results differ among individuals. Some recommend combining it with finasteride for better long-term results, though OP chooses to use only minoxidil and accepts the possibility of going bald.

community The PP405 news are the best news we've had in the last 20 or more years.

599 upvotes 1 year ago

PP405 significantly improves hair density, outperforming finasteride, with 31% of users seeing over 20% improvement in four weeks. Concerns exist about its availability, cost, and long-term effects.

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Learn

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learn Overview

learn Beta Polypeptide

learn Fluocinolone

Can long-term use of Fluocinolone affect the health of hair follicles? There is no long-term human trial measuring what topical fluocinolone does to hair follicles; what is documented is the label's warning about skin atrophy with prolonged use, plus a 2007 mouse study suggesting hair-follicle bulge stem cells do not repair steroid-induced atrophy.

learn Hydrocortisone

What are the risks of long-term hydrocortisone use on the scalp? Dermatology reviews describe long-term topical corticosteroid use as causing skin thinning, delayed healing, greater infection risk, possible suppression of the body's own cortisol, and rebound flares on stopping — and over-the-counter hydrocortisone labels limit use to about a week without a doctor's direction.

learn Osteopontin

Is Osteopontin safe to use long-term in hair growth products? Osteopontin's long-term safety in hair growth products is not yet established.

learn Rapamycin

Is rapamycin safe for long-term use on the scalp, or should it be used in cycles? There is no published human trial of topical rapamycin on the scalp, so nobody knows whether long-term use is safe; the idea of cycling it comes from community practice and theory, not from any study or guideline, and rapamycin for hair loss is an off-label use of a prescription immunosuppressant.

learn Azelaic Acid

Is azelaic acid safe to use daily long-term? Azelaic acid has a long tolerability record on facial skin in acne and rosacea, where side effects are local and usually mild — but for daily long-term scalp use the only trial is one six-month study in 26 women, in which mild itching was the main adverse effect, so long-term scalp safety is not established.

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