research Fluorescence Activated Cell Sorting of CK15 Cells in Canine Hair Follicular Tissue
The study investigated the multipotent stemness of canine hair follicle stem cells (cHFSCs) using Fluorescence Activated Cell Sorting (FACS) and immunostaining techniques. Canine hair follicular cells were cultured in Williams’E media with growth factors, and 18.28% of the cells were found to be positive for the CK15 marker, indicating their stemness. The positive expression of CK15, observed with DAPI counterstaining, suggested that cells in the canine hair follicular bulge region acted as precursors involved in the hair cycle.
