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This study designed a micromold template for creating controlled-sized, multicellular tumor spheroids, finding that HEK-293 cells formed consistent spheroids with high circularity and viability after 7 days, influenced by cell seeding density and treatment conditions.

This study found that 3D microtissue models of dermal papilla cells can enhance the ability to induce hair-follicle neogenesis in vivo, offering potential for controlled cell production in follicle regeneration.

This study demonstrated that combining the Wnt/β-catenin activator CHIR99021 with 3D spheroid culture effectively enriched hair follicle formation in reconstituted human skin.

This study found that TGF-β2 may enhance collagen expression and spheroid formation in human dermal papilla cells, which could improve their hair inductivity and maintenance.

This study found that elevated STAT5 levels are linked to improved hair-inducing capabilities in human dermal papilla cells, and reducing STAT5 impairs hair follicle induction.