Wound Healing Efficacy of Cellulose Hydrogel on ICR Mice: A Morphoanatomical, Histological, and Genomic Study

This study investigated the wound healing efficacy of NIMO-CH, a cellulose hydrogel derived from nipa fronds and enriched with Lacticaseibacillus paracasei BCRC-16100, on ICR mice with excisional wounds. The treatments compared were no treatment, NIMO-CH, and DuoDERM. Both NIMO-CH and DuoDERM achieved 100% wound closure, with NIMO-CH-treated wounds showing complete healing with hair growth by days 18-20, while untreated wounds healed by day 20. Minimal scarring was observed in both NIMO-CH and DuoDERM groups. Histological analysis showed similar healing processes, and genome sequencing identified genes sodA and gsiC in Lacticaseibacillus paracasei BCRC-16100, which are important for wound healing. The results suggest that NIMO-CH could be an effective alternative for wound care applications.