Ultrasound and Microwave Assisted Extraction of Luteolin from Eclipta Prostrata

The study established an efficient method for extracting luteolin (LU) from Eclipta prostrata using ultrasound assisted microwave extraction (UAME). The optimized extraction process involved a specific ratio of 80% ethanol, hydrochloric acid, and plant material, with microwave power set at 40 W and an extraction time of 3 × 3 minutes. This method yielded 0.690 mg/g of LU and was found to be more efficient, less time-consuming, and less labor-intensive compared to heat reflux extraction (HRE) and reflux acid extraction (RAE). UAME significantly reduced extraction time and simplified the process of extracting LU from E. prostrata.