The Effects of Spermidine on Functional and Transcriptomic Markers in Human Primary Keratinocytes

This in vitro study explored the effects of spermidine (SPD) on human primary keratinocytes, revealing that 10 µM SPD increased cell viability by 9% and significantly altered 162 transcripts, enhancing pathways related to protein deubiquitination and cell cycle processes. Notably, the HR gene, important for hair follicle formation, was up-regulated, and pan-keratin protein content increased by 60%. Additionally, mitochondrial membrane potential improved by 84% with 1 µM SPD. These findings suggest SPD positively influences keratinocyte homeostasis and may enhance skin health and hair growth, although further research is needed to explore these effects under stress conditions.