Streamlined Intravital Imaging Approach for Long-Term Monitoring of Epithelial Tissue Dynamics on an Inverted Confocal Microscope

This study introduces a custom-designed, 3D-printed microscope stage insert for inverted confocal microscopes, enabling streamlined long-term intravital imaging of epithelial tissue dynamics, specifically in the ear skin of live transgenic mice. This innovation addresses the accessibility barrier posed by traditional upright multiphoton microscopes, making intravital microscopy more accessible to researchers. The invention can be customized for different microscope brands and adapted for imaging various organ systems, enhancing the study of live cell dynamics in both normal and disease contexts, and potentially aiding in the development of clinical interventions.