Standard DNA Methylation Analysis in Mouse Epidermis: Bisulfite Sequencing, Methylation-Specific PCR, and 5-Methyl-Cytosine Immunological Detection

October 2013 in “ Methods in molecular biology ”

Jesús Espada, Elisa Carrasco, María I. Calvo-Sánchez

DNA methylation bisulfite sequencing methylation-specific PCR 5-methyl-cytosine epidermis hair follicle stem cells 5mC

TLDR These methods help understand DNA changes in mouse skin.

The document described standard procedures for analyzing DNA methylation in mouse epidermis, focusing on bisulfite sequencing, methylation-specific PCR, and 5-methyl-cytosine (5mC) immunodetection. These methods were crucial for understanding DNA methylation dynamics, which played a significant role in embryo development and adult tissue homeostasis. The study used mouse skin and hair follicle stem cell niches as model tissues to illustrate these techniques.

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research Manipulation of Stem Cell Proliferation and Lineage Commitment: Visualization of Label-Retaining Cells in Wholemounts of Mouse Epidermis

421 citations , September 2003 in “Development”

Stem cell behavior varies with stimuli, and lineage changes can happen without affecting stem cell division.

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