Standard DNA Methylation Analysis in Mouse Epidermis: Bisulfite Sequencing, Methylation-Specific PCR, and 5-Methyl-Cytosine Immunological Detection

    October 2013 in “ Methods in molecular biology ”
    Jesús Espada, Elisa Carrasco, María I. Calvo-Sánchez
    TLDR These methods help understand DNA changes in mouse skin.
    The document described standard procedures for analyzing DNA methylation in mouse epidermis, focusing on bisulfite sequencing, methylation-specific PCR, and 5-methyl-cytosine (5mC) immunodetection. These methods were crucial for understanding DNA methylation dynamics, which played a significant role in embryo development and adult tissue homeostasis. The study used mouse skin and hair follicle stem cell niches as model tissues to illustrate these techniques.
    Discuss this study in the Community →