Standard DNA Methylation Analysis in Mouse Epidermis: Bisulfite Sequencing, Methylation-Specific PCR, and 5-Methyl-Cytosine Immunological Detection

    October 2013 in “ Methods in molecular biology ”
    Jesús Espada, Elisa Carrasco, María I. Calvo-Sánchez
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    TLDR These methods help understand DNA changes in mouse skin.
    The document described standard procedures for analyzing DNA methylation in mouse epidermis, focusing on bisulfite sequencing, methylation-specific PCR, and 5-methyl-cytosine (5mC) immunodetection. These methods were crucial for understanding DNA methylation dynamics, which played a significant role in embryo development and adult tissue homeostasis. The study used mouse skin and hair follicle stem cell niches as model tissues to illustrate these techniques.
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