Standard DNA Methylation Analysis in Mouse Epidermis: Bisulfite Sequencing, Methylation-Specific PCR, and 5-Methyl-Cytosine Immunological Detection

October 2013 in “ Methods in molecular biology ”

Jesús Espada, Elisa Carrasco, María I. Calvo-Sánchez

DNA methylation bisulfite sequencing methylation-specific PCR 5-methyl-cytosine epidermis hair follicle stem cells 5mC

TLDR These methods help understand DNA changes in mouse skin.

The document described standard procedures for analyzing DNA methylation in mouse epidermis, focusing on bisulfite sequencing, methylation-specific PCR, and 5-methyl-cytosine (5mC) immunodetection. These methods were crucial for understanding DNA methylation dynamics, which played a significant role in embryo development and adult tissue homeostasis. The study used mouse skin and hair follicle stem cell niches as model tissues to illustrate these techniques.

View this study on digital.csic.es →

Discuss this study in the Community →

Related Community Posts Join

6 / 132 results

community Why is dht bad for scalp hair but good for hair on the rest of body?

in Satire 109 upvotes 1 year ago

The conversation discusses why DHT (dihydrotestosterone) negatively affects scalp hair but promotes growth elsewhere on the body. Various opinions include genetic predispositions, differences in hair follicle reactions to DHT, and the potential role of Omega-3 in reducing inflammation and promoting hair health.

Compressed part of research of theory of androgenic/anabolitic balance. AGA h-responders analytic. Theory of physio-metabolitic method of anti AGA treatment

community Compressed part of research of theory of androgenic/anabolitic balance. AGA h-responders analytic. Theory of physio-metabolitic method of anti AGA treatment

in Research 862 upvotes 3 months ago

The treatment for androgenetic alopecia involves using finasteride and minoxidil with intense exercise and cold exposure to boost metabolism and reduce androgenic effects, potentially leading to hair regrowth. This approach may activate biological pathways for improved hair and overall health.

community The Real Cause Of Androgenetic Alopecia

in Research/Science 673 upvotes 4 months ago

Androgenetic alopecia is caused by DHT affecting hair growth. Finasteride and minoxidil are used to manage hair loss by blocking DHT and promoting hair growth.

community So we have 20k dollars human like machines available but no new effective treatment for hairloss since finasteride :)

in Chat 237 upvotes 4 months ago

There have been no new effective hair loss treatments since finasteride, despite technological advancements. Current treatments include finasteride, minoxidil, and RU58841, with ongoing challenges and potential future solutions in research.

community Doctor says i shouldn't take fin because its "hormonal"

in Finasteride/Dutasteride 103 upvotes 6 months ago

A user in Turkey was advised by a dermatologist to avoid finasteride due to its hormonal effects and was prescribed minoxidil, shampoo, and vitamins instead. The user is considering seeking another doctor's opinion or buying finasteride directly from a pharmacy.

community If You Have DUPA, PLEASE READ THIS: Everyone Should Be Scalp Biopsied

in Research 830 upvotes 6 months ago

Scalp biopsies are crucial for diagnosing hair loss conditions like Diffuse Unpatterned Alopecia (DUPA) and retrograde hair loss, as treatments like finasteride and dutasteride may not be effective if other conditions are present. Combining PPAR-GAMMA agonists with retinoids could improve treatments for conditions like Lichen Planopilaris.