Screening and Culture of Human Vaginal Mucosa Stem Cells

    January 2008 in “ Zhongguo zuzhi gongcheng yanjiu yu linchuang kangfu ”
    Huinan Weng, Yifeng Wang, Fenghua Liu
    TLDR Vaginal mucosa stem cells can be effectively isolated and cultured using specific methods.
    This study aimed to identify effective methods for isolating, identifying, and culturing human vaginal mucosa stem cells (VMSCs). Conducted at the Third Affiliated Hospital of Guangzhou Medical College, the research involved five patients and utilized trypsin-collagenase digestion for cell harvesting. VMSCs were selected by adhesion to type Ⅳ collagen and analyzed using flow cytometry. The study found significant differences in cell cycle phases and marker expression between VMSCs and primary vaginal mucosa cells. The optimal culture condition was using feeder layer and epidermal total culture media, which resulted in the highest colony forming efficiency and CK19 positive rate, and the lowest CK10 positive rate. VMSCs could be passaged over 15 times, indicating successful maintenance of their characteristics.
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