Decision Letter: Sphingosine 1-Phosphate-Regulated Transcriptomes in Heterogeneous Arterial and Lymphatic Endothelium of the Aorta
November 2019
TLDR S1PR1 helps control inflammation in blood vessel cells by affecting gene activity differently in various cell types and locations.
The study explored the role of sphingosine 1-phosphate receptor (S1PR1) signaling in aortic endothelial cells, revealing that S1PR1 signals through both ligand-dependent and ligand-independent pathways in distinct endothelial cell subsets. It highlighted endothelial cell heterogeneity and the influence of cell lineage on the unique transcriptome of arterial endothelial cells, rather than flow signaling. The research also examined GFP expression in endothelial cells, noting variations with age and specific markers, and extended its analysis to embryonic stages, identifying differences in marker expression between embryonic and adult aortae. While the study was technically rigorous and biologically impactful, it noted concerns about the reporter line and bioinformatics data, suggesting areas for further analysis.
