Development And Validation Of A RP-HPLC Method For The Simultaneous Determination Of Spironolactone And Hydrochlorothiazide In Pure And Pharmaceutical Dosage Form
January 2023
in “
Zenodo (CERN European Organization for Nuclear Research)
”
TLDR A new accurate and reliable method was developed to measure Spironolactone and Hydrochlorothiazide together in medicines.
A new RP-HPLC method was developed and validated for the simultaneous estimation of Spironolactone and Hydrochlorothiazide in pharmaceutical dosage forms. The method utilized an Inertsil C18 column with a mobile phase of Methanol and Phosphate buffer, achieving retention times of 1.688 mins and 3.282 mins for the two compounds. The % purity was 99.86%, and the method showed excellent linearity with correlation coefficients of 0.999 for both drugs. The % recovery was approximately 100%, and the method demonstrated high precision, robustness, and repeatability. The LOD and LOQ values were determined for both drugs, confirming the method's suitability for routine analysis in pharmaceutical applications.
