Development And Validation Of A RP-HPLC Method For The Simultaneous Determination Of Spironolactone And Hydrochlorothiazide In Pure And Pharmaceutical Dosage Form

    January 2023 in “ Zenodo (CERN European Organization for Nuclear Research) ”
    H. Padmalatha
    TLDR A new accurate and reliable method was developed to measure Spironolactone and Hydrochlorothiazide together in medicines.
    A new RP-HPLC method was developed and validated for the simultaneous estimation of Spironolactone and Hydrochlorothiazide in pharmaceutical dosage forms. The method utilized an Inertsil C18 column with a mobile phase of Methanol and Phosphate buffer, achieving retention times of 1.688 mins and 3.282 mins for the two compounds. The % purity was 99.86%, and the method showed excellent linearity with correlation coefficients of 0.999 for both drugs. The % recovery was approximately 100%, and the method demonstrated high precision, robustness, and repeatability. The LOD and LOQ values were determined for both drugs, confirming the method's suitability for routine analysis in pharmaceutical applications.
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