Investigation of ROP GTPase Activity and Cytoskeleton Dynamics During Tip Growth in Root Hairs and Pollen Tubes
January 2023
in “
Methods in molecular biology
”
ROP GTPase cytoskeleton dynamics tip growth root hairs pollen tubes calcium gradient actin dynamics vesicle trafficking microtubules organelle movement growth directionality spatiotemporal localization cortical microtubule organization F-actin dynamics cytoskeleton calcium actin vesicle organelle growth direction localization microtubule organization F-actin
TLDR ROP GTPase helps control the growth of pollen tubes and root hairs by managing cell structure and movement.
The document discusses the role of Rho family ROP GTPase in the growth of pollen tubes and root hairs, which are typical tip-growing cells in plants. The study reveals that ROP GTPase, when periodically activated, works in coordination with the tip-focused calcium gradient to regulate actin dynamics and vesicle trafficking, which are crucial for cell growth. The study also highlights the involvement of microtubules in organelle movement and growth directionality. The methods for analyzing the spatiotemporal localization and activity of ROP, cortical microtubule organization, and F-actin dynamics in pollen tubes and/or root hairs are described.
