Progenitor Cells Expressing Nestin, a Neural Crest Stem Cell Marker, Differentiate Into Outer Root Sheath Keratinocytes
July 2019
in “
Veterinary Dermatology
”
Research cited in this study
8 / 8 results
research Hair Follicle Dermal Stem Cells Regenerate the Dermal Sheath, Repopulate the Dermal Papilla, and Modulate Hair Type
Hair follicle dermal stem cells are key for regenerating parts of the hair follicle and determining hair type.
research The contribution of stem cells to epidermal and hair follicle tumours in the dog
This study investigated the expression of stem cell markers keratin 15 (K15) and nestin in 44 canine follicular tumours and 30 squamous cell carcinomas to understand their role in tumourigenesis. K15 was found to be a reliable marker for studying stem cells in hair follicle tumours, with strong expression in trichoblastomas and variable expression in tricholemmomas and trichoepitheliomas. In contrast, nestin was not an optimal marker due to its faint expression. The presence of hair follicle stem cells in the bulge region and the loss of K15 expression in squamous cell carcinomas suggested a potential role in the development and malignant transformation of these tumours.
research Multipotent nestin-expressing stem cells capable of forming neurons are located in the upper, middle and lower part of the vibrissa hair follicle
The study demonstrated that multipotent, nestin-expressing stem cells were present throughout the vibrissa hair follicle, with the greatest differentiation potential in the upper part. These stem cells, identified using a transgenic mouse model, could differentiate into neurons, glia, keratinocytes, smooth muscle cells, and melanocytes. The upper part of the follicle produced a large number of spheres from nestin-expressing cells, which differentiated into various cell types, suggesting potential applications for nerve and spinal cord repair.
research Canine Follicle Stem Cell Candidates Reside in the Bulge and Share Characteristic Features with Human Bulge Cells
Canine hair follicles have stem cells similar to human hair follicles, useful for studying hair disorders.
research Canine hair‐follicle keratinocytes enriched with bulge cells have the highly proliferative characteristic of stem cells
research Multipotent nestin-positive, keratin-negative hair-follicle bulge stem cells can form neurons
The study demonstrated that nestin-positive, keratin-negative stem cells from the hair-follicle bulge area in transgenic mice could differentiate into various cell types, including neurons, glia, keratinocytes, smooth muscle cells, and melanocytes in vitro. These stem cells, marked by ND-GFP and CD34 positivity, were shown to be in a relatively undifferentiated state, indicating their pluripotency. Additionally, when transplanted into the subcutis of nude mice, these cells could differentiate into neurons. The findings suggested that these hair-follicle bulge-area stem cells might serve as an accessible and autologous source of multipotent stem cells for therapeutic applications.
research Nascent Blood Vessels in the Skin Arise from Nestin-Expressing Hair-Follicle Cells
Hair follicle cells can create new blood vessels in the skin.
research Nestin expression in hair follicle sheath progenitor cells
The study demonstrated that nestin, an intermediate filament protein, was expressed in progenitor cells of the hair follicle outer-root sheath, particularly during different phases of the hair cycle. Using nestin-GFP transgenic mice, researchers observed that nestin-expressing cells were located in the hair follicle bulge during the telogen and early anagen phases, and in both the bulge and upper outer-root sheath during mid- and late anagen phases. Immunohistochemical staining confirmed the colocalization of nestin with other proteins in these areas, supporting the conclusion that nestin-expressing cells in the hair follicle bulge are progenitors of the outer-root sheath. The study suggested a potential relationship between neural stem cells and hair follicle stem cells due to the expression of nestin in both cell types.
Related Research
1 / 1 resultsresearch Nestin expression in hair follicle sheath progenitor cells
The study demonstrated that nestin, an intermediate filament protein, was expressed in progenitor cells of the hair follicle outer-root sheath, particularly during different phases of the hair cycle. Using nestin-GFP transgenic mice, researchers observed that nestin-expressing cells were located in the hair follicle bulge during the telogen and early anagen phases, and in both the bulge and upper outer-root sheath during mid- and late anagen phases. Immunohistochemical staining confirmed the colocalization of nestin with other proteins in these areas, supporting the conclusion that nestin-expressing cells in the hair follicle bulge are progenitors of the outer-root sheath. The study suggested a potential relationship between neural stem cells and hair follicle stem cells due to the expression of nestin in both cell types.