Peer Review of a Novel Control of Human Keratin Expression: Cannabinoid Receptor 1-Mediated Signaling Down-Regulates the Expression of Keratins K6 and K16 in Human Keratinocytes In Vitro and In Situ

The study investigated the role of cannabinoid receptor 1 (CB1) in regulating keratin expression, specifically K6 and K16, in human keratinocytes using normal skin organ culture and a keratinocyte line model. The research demonstrated that CB1 agonists reduced the expression of these keratins, suggesting potential therapeutic applications for hyperproliferative skin conditions like psoriasis. However, the study faced issues such as unclear differentiation between in vivo and cultured skin keratin expression levels and a lack of direct evidence linking decreased proliferation to reduced K6 expression. The discussion and conclusion needed refinement to focus more on the main findings and their implications.