Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues
December 1988
in “
Differentiation
”
Research cited in this study
11 / 11 resultsresearch Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues II. Concomitant and mutually exclusive synthesis of trichocytic and epithelial cytokeratins in diverse human and bovine tissues (hair follicle, nail bed and matrix, lingual papilla, thymic reticulum)
research Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles
research Keratins of the human hair follicle: “Hyperproliferative” keratins consistently expressed in outer root sheath cells in vivo and in vitro
research Cultivation of Murine Hair Follicles as Organoids in a Collagen Matrix
research Acidic and Basic Hair/Nail (Hard) Keratins: Their Colocalization in Upper Cortical and Cuticle Cells of the Human Hair Follicle and Their Relationship to Soft Keratins
Hair and nail cells share similar proteins, indicating a common differentiation pathway.
research The complement of native α-keratin polypeptides of hair-forming cells: A subset of eight polypeptides that differ from epithelial cytokeratins
research Cell Differentiation in Human Anagen Hair and Hair Follicles Studied with Anti-Hair Keratin Monoclonal Antibodies
research Immunological characteristics and histological distribution of human hair fibrous proteins studied with anti-hair keratin monoclonal antibodies HKN-2, HKN-4, and HKN-6.
The study used monoclonal antibodies HKN-2, HKN-4, and HKN-6 to investigate the immunological characteristics of human hair fibrous proteins. HKN-2 and HKN-4 marked both hair and epidermal fibrous proteins, while HKN-6 was specific to hair fibrous proteins. Immunohistochemical analysis showed that all three antibodies stained the keratogenous zone of anagen hairs, with HKN-6 being specific to hair and not reacting with other skin components or tissues. HKN-2 reacted with skin epithelial tissues but was limited to the skin, whereas HKN-4 showed broad crossreactivity with various epithelial cells. The findings suggested that some hair fibrous proteins were specific to hair cells, while others crossreacted with proteins in other epithelial cells, indicating the potential of these antibodies in studying epithelial cell differentiation.
research Expression of epidermal keratins and filaggrin during human fetal skin development.
The study analyzed keratins and filaggrin in over 50 samples of human embryonic and fetal skin, identifying four periods of epidermal development: embryonic, stratification, follicular keratinization, and interfollicular keratinization. Keratins of 50 and 58 kD were consistently present, while 56.5 and 67 kD keratins appeared during stratification and increased with development. Filaggrin was first detected at 15 weeks, associated with keratinizing cells around hair follicles, and later in the granular and cornified layers. The findings indicated a relationship between protein expression and epidermal morphologic changes, with keratin expression marking tissue commitment to stratification and keratinization, and no unique "fetal" keratins were identified.
research Different keratin polypeptides in epidermis and other epithelia of human skin: a specific cytokeratin of molecular weight 46,000 in epithelia of the pilosebaceous tract and basal cell epitheliomas.
The study analyzed cytokeratin polypeptides in human skin, focusing on the epidermis, pilosebaceous tract, and eccrine sweat glands, using gel electrophoresis and antibody binding. It found that the pilosebaceous tract shared some keratins with interfollicular epidermis but had a unique acidic cytokeratin of molecular weight 46,000. This keratin was also prominent in basal-cell epitheliomas, showing a similarity between these tumors and hair-follicle epithelium. The study concluded that different skin epithelia could be distinguished by their specific cytokeratin patterns, and the cytoskeleton of basal-cell epitheliomas was related to that of the pilosebaceous tract.
research Keratin cytoskeletons in epithelial cells of internal organs
The study used an antiserum against human epidermal keratins to detect keratins in various rabbit and human tissues through indirect immunofluorescence. Strong keratin staining was observed in stratified squamous epithelia, epidermal appendages, and certain epithelial cells of the intestinal, urinary, female genital, and respiratory tracts, as well as in the fine ductal system of the pancreas and submaxillary gland. No staining occurred in fibroblasts, muscle, cartilage, blood vessels, nerve tissue, or kidney cells. The findings indicated that keratins form a cytoskeleton in epithelial cells, providing structural strength, and that keratin staining can help determine the origin of cell lines.
Related Research
7 / 7 resultsresearch Functional analysis of keratin components in the mouse hair follicle inner root sheath
Keratin in mouse hair follicles is complex and plays specific roles.
research ‘Hard’ and ‘soft’ principles defining the structure, function and regulation of keratin intermediate filaments
This review explored the structure, function, and regulation of keratin intermediate filaments (IFs), highlighting both established ('hard') and emerging ('soft') principles. It discussed the discovery of novel keratin genes, the mechanics of keratin filaments, and the functional redundancy revealed by gene knockouts. The study also examined the role of keratin mutations in various diseases and the regulation of keratin through interactions with other proteins. The field was noted to be rapidly advancing, driven by transgenic models and new insights into keratin's protective roles in cellular stress responses.
research A Novel Type II Cytokeratin, mK6irs, is Expressed in the Huxley and Henle Layers of the Mouse Inner Root Sheath
The study identified a novel type II cytokeratin, mK6irs, specifically expressed in the Huxley and Henle layers of the mouse inner root sheath (IRS) during the anagen phase of hair growth. This 57 kDa protein showed homology with other cytokeratins and was confirmed through various analyses to be present only during specific phases of the hair cycle. The research suggested that mK6irs played a role in the differentiation and structure of the IRS, contributing to the understanding of hair follicle biology. Additionally, the study proposed that mK6irs could be important for understanding hereditary hair diseases, as mutations in keratins are linked to conditions like cicatricial alopecia and monilethrix.
research Study on the distribution of hair keratin in normal hair follicles by immunohistochemical staining
Different keratins in hair follicles can help identify hair tumor origins.
research Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles
research Keratins of the human hair follicle: “Hyperproliferative” keratins consistently expressed in outer root sheath cells in vivo and in vitro
research Immunohistochemical staining characteristics of epidermal appendages (hair follicles and eccrine sweat glands) to anti-epidermal keratin antisera
The study investigated the immunohistochemical staining characteristics of epidermal appendages, specifically hair follicles and eccrine sweat glands, using antisera against keratin proteins. The research found that in hair follicles, the medulla, cortex, and inner root sheath were positively stained with both antisera only in the keratogenous zone, while the outer root sheath showed variable staining with 64KA. Eccrine glands exhibited a heterogeneous staining pattern in the secretory portion, contrasting with the homogeneous staining of ductal portions. In eccrine poroma, tumor cells were uniformly stained with TKA, but showed mixed staining with 64KA, indicating varied levels of keratinization among the cells.