Optimized Depilation Method and Comparative Analysis of Hair Growth Cycle in Mouse Strains

The study introduces an optimized depilation method for synchronizing hair growth cycles in mouse models, specifically C57BL/6J, B6129F1, and Sv129 strains, which significantly reduces skin injury and enhances the reliability of hair growth research. The new protocol involves shaving, depilating with cold wax, cleaning with mineral oil, and applying protective sprays, reducing wound frequency from 100% to 50%. The research, conducted on 53 mice, found no significant differences in depilation outcomes among the strains. It also revealed that while all strains follow a similar hair cycle timeline, Sv129 and B6129F1 mice have higher hair density due to increased hair follicle number and size during anagen. C57BL/6J mice are preferred for hair research due to their contrast between lighter skin and darker hair.