A Novel Crosslinking Approach for Biomanufacturing of a Collagen-Based Skin Dermal Template

    December 2024 in “ Macromolecular Bioscience ”
    Weng Wan Chan, Kaushik Roy, Bach Quang Le, Hariharan Ezhilarasu, Xiaoqian Zhang, Ryan G. Lim, Avinanda Banerjee, Moni Abraham Kuriakose, Kimmie Ng, Priya Murugan, Chun Ting Goh, Weibiao Zhou, May Win Naing, Srikala Raghavan, Deepak Choudhury
    TLDR The new collagen template speeds up production and supports skin healing without harmful reactions.
    The study introduces a novel crosslinking method for collagen-based skin dermal templates, aimed at improving treatment for third-degree burns. This new approach uses UV irradiation followed by glutaraldehyde (GA) crosslinking, which reduces the crosslinking time from 48 to 24 hours compared to traditional methods. The collagen template demonstrated superior support for human dermal fibroblast cell line WS-1 proliferation compared to the commercial Integra template after 2 weeks in culture. In vivo tests on mouse models showed similar skin wound regeneration levels between the new sponge and Integra templates, without causing abnormal angiogenic or immune responses. This method presents a promising alternative for producing collagen sponge scaffolds.
    Discuss this study in the Community →

    Research cited in this study

    1 / 1 results