Multifunctional NIR-Triggered Nanozyme-Based Microneedles for Synergistic Eradication of MRSA and Enhanced Wound Healing

The study presents a novel approach to treating MRSA-infected wounds using multifunctional near-infrared (NIR)-triggered nanozyme-based microneedles (MN@CTB). These microneedles incorporate CTB nanozymes, which combine nitric oxide (NO) prodrugs with a phenolic network of Cu2+-tannic acid. The treatment effectively eradicates MRSA through the synergistic effects of NIR-triggered NO release and local hyperthermia. Additionally, MN@CTB exhibits strong antioxidant, anti-inflammatory, and angiogenic properties by activating the Nrf-2/HO-1 signaling pathways and inhibiting the NF-κB signaling pathway. It also enhances angiogenesis by upregulating soluble guanylate cyclase (sGC) and activating the PKG signaling pathway. Proteomic analysis reveals that MN@CTB reprograms the wound microenvironment by inhibiting NF-κB and activating VEGF/TGF-β pathways, offering a promising strategy for enhancing wound healing in MRSA infections.