Live-Cell Imaging and Analysis of Germline Stem Cell Mitosis in Caenorhabditis Elegans

    January 2022 in “ BIO-PROTOCOL ”
    Réda Zellag, Yifan Zhao, Abigail Gerhold
    Studysummary This article provides a protocol for live imaging of C. elegans germline stem cells, highlighting their potential for studying mitosis and other cell processes, but does not report new experimental findings.
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    The study presented a protocol for live-cell imaging and analysis of germline stem cell mitosis in Caenorhabditis elegans, highlighting the accessibility of C. elegans GSCs for in situ imaging. The protocol was optimized for imaging and analyzing mitotic GSCs and included tools for processing large datasets. It was adaptable for visualizing dividing cells or other subcellular processes in C. elegans across various developmental stages. Additionally, the image analysis pipeline could be applied to study mitosis in other cell types and model organisms.
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