Long-Term Live Imaging of the Drosophila Adult Midgut Reveals Real-Time Dynamics of Cell Division, Differentiation, and Loss

    February 2018 in “ bioRxiv (Cold Spring Harbor Laboratory) ”
    Judy Lisette Martin, Erin Sanders, Paola Moreno-Roman, Shruthi Balachandra, Xinxin Du, Leslie Ann Jaramillo Koyama, Lucy Erin O’Brien
    TLDR Researchers can now observe live cell processes in the Drosophila midgut for extended periods.
    The study presented a novel platform for extended live imaging of the adult Drosophila midgut, allowing researchers to observe cell division, differentiation, and loss in real time. By creating a window in a living Drosophila, the midgut could be imaged while remaining intact and functional, enabling imaging sessions of 12-16 hours. This method revealed new cell behaviors, such as the dynamic re-orientation of mitotic stem cells, delayed Notch activation in daughter cells, and enterocyte extrusion through a pulsatile cadherin ring. This platform provided a new way to understand the dynamics of midgut organ renewal.
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