LC/MS Analysis of Stratum Corneum Lipids: Ceramide Profiling and Discovery
March 2011
in “
Journal of Lipid Research
”
TLDR A new, quick method was developed to analyze skin lipids, discovering a new ceramide subclass.
The study developed a novel LC/MS method for analyzing ceramides (CERs) in the stratum corneum (SC) of human skin, identifying all 11 known CER subclasses and discovering a new subclass, CER [EOdS]. The method was quick, robust, and required minimal sample preparation, validated using three types of SC samples: human dermatomed skin, tape-stripped human SC, and full-thickness skin explants, all showing slight differences in CER profiles. The method offered advantages over previous techniques by reducing analysis time and sample preparation steps while maintaining high sensitivity, utilizing normal phase liquid chromatography (NPLC) with atmospheric pressure chemical ionization (APCI) for efficient separation and shorter analysis times.
