LC/MS Analysis of Stratum Corneum Lipids: Ceramide Profiling and Discovery

    March 2011 in “ Journal of Lipid Research ”
    Jeroen van Smeden, Louise Hoppel, Rob van der Heijden, Thomas Hankemeier, Rob J. Vreeken, Joke A. Bouwstra
    Studysummary This study reports a novel LC/MS method that separates and analyzes all known ceramide subclasses in human stratum corneum, identifying a new subclass, CER [EOdS], with minimal sample preparation.
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    The study from June 2011 developed a novel LC/MS method for analyzing ceramides in the stratum corneum, identifying all 11 known ceramide subclasses and discovering a new subclass, CER [EOdS]. This method was quick, robust, and required minimal sample preparation, showing slight differences in ceramide profiles among various human skin samples. The method offered advantages over previous techniques, including reduced analysis time and higher sensitivity, making it suitable for detailed ceramide profiling in skin research. The study concluded that this new method is beneficial for future ceramide analysis.
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