Human Hair-Derived Keratin: Extraction and Comparative Assessment

January 2025 in “ International Journal of Molecular Biology and Biochemistry ”

Sonia Angeline M, N Pratiksha, Govardhan Palekar, G Hemanth, Santosh Kumar Sinha, Mudassira Zuha Anjum

keratin human hair Shindai method denaturing agents UV-Spectrophotometry SDS-PAGE α-keratin

TLDR The Shindai method effectively extracts keratin from human hair, with potential uses in bioplastics and biofertilizers.

The study investigates the extraction of keratin from human hair using the Shindai method, which involves strong denaturing agents. The research found that female hair yields more keratin compared to male hair. The extracted keratin was analyzed using UV-Spectrophotometry and SDS-PAGE, confirming the presence of α-keratin with a molecular weight range of 40-70 KDa. The study concludes that the Shindai method is effective for extracting keratin, which has potential applications in bioplastics and biofertilizers.

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