Promising Protocol for In Vivo Experiments with Betulin

This study presents a promising method for administering betulin in in vivo experiments, addressing its water insolubility by dissolving it in ethanol and introducing it into acylglycerols, with olive oil and food-grade lard identified as suitable carriers. The research found that betulin has a specific distribution in the organs of Wistar rats and demonstrated a significant synergistic effect on solid Ehrlich adenocarcinoma tumors when combined with Namitecan in NMRI mice. The study concludes that the method of administration and analytical procedure could enhance the reliability of future studies on betulin and similar compounds.