Histocultures And Their Use

Histocultures, which are three-dimensional tissues maintained in growth medium, were used to retain in vivo-like phenotypes, unlike monolayer cultures. They had various applications, including testing patient tumors for drug sensitivity to tailor individualized cancer treatments, as demonstrated in clinical trials. Histocultures of skin were particularly significant for studying hair growth and screening drugs affecting hair growth, leading to the discovery of hair follicle-targeting cancer liposomes for gene delivery. Additionally, histocultures of lymphoid organs were developed to study antibody production and viral effects on immune cells, while stem cell growth and differentiation were also explored using this method.