Authentication of Herbs by Physiological and Morphological Studies Through HPTLC, ICP-MS, UV Spectroscopy, Genetic Engineering and PCR
December 2025
in “
Universities Journal of Phytochemistry and Ayurvedic Heights
”
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Studysummary This study highlights a comprehensive approach for authenticating and assessing the quality of herbal medicines using a combination of physiological, morphological, and molecular techniques, including HPTLC, ICP-MS, UV spectroscopy, and DNA barcoding, to ensure the standardization of herbal medicinal products, particularly Eclipta alba.
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The article discusses the need for stringent quality control measures in the global herbal medicinal products market to address issues like adulteration and contamination. It highlights research on Eclipta alba (Bhringaraj) using a multi-disciplinary approach that combines physiological and morphological studies, HPTLC, ICP-MS, UV Spectroscopy, and genetic engineering techniques like DNA barcoding/PCR. This integrated strategy offers a comprehensive three-tiered confirmation of botanical origin, chemical profile, and safety (heavy metal) profile, which is crucial for the standardization and regulation of herbal medicines.