Application of Phytochemical Screening and a Combined FTIR Spectroscopy and Principal Component Analysis for Effective Discrimination of Two Varieties of Eclipta Alba (L.) Hassk.

    N. Geetha, Vadakkenchery Salimudheen Hansiya, Palanisamy Uma Maheswari
    TLDR The two Eclipta alba varieties can be distinguished by their chemical differences.
    The study aimed to effectively discriminate between two varieties of Eclipta alba, White Karisalanganni (WK) and Manjal Karisalanganni (MK), using phytochemical screening and a combination of FTIR spectroscopy and principal component analysis (PCA). The research found that the MK variety exhibited C=H and C=O stretches, indicating the presence of alcohol and ester functional groups, while the WK variety showed an alkyne group in its chloroform extract. These chemical differences, although slight, were significant enough to distinguish the two varieties. The findings supported the use of these methods for accurate authentication of the plant varieties for research, food, and pharmaceutical applications.
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