Determination of Finasteride in Human Plasma by LC-MS and Its Pharmacokinetics
January 2004
in “
Zhongguo xin yao zazhi
”
TLDR The method accurately measures finasteride in plasma, and the test tablets are as effective as the reference ones.
The study established a simple, sensitive, and rapid LC-MS method to determine finasteride concentration in human plasma and examined the pharmacokinetics of domestic finasteride tablets. Using a sample of 20 healthy subjects, the study found that the assay had a linear range from 1.04 to 165.76 ng/mL with a correlation of 0.9996, a detection limit of 0.4 ng/mL, and a quantitation limit of 1.0 ng/mL. The average extraction recovery exceeded 93%, and the intra-day and inter-day RSD were both under 15%. The pharmacokinetic parameters of the test tablets were bioequivalent to the reference tablets, with a relative bioavailability of 96.8% ± 9.3%.