A Femtosecond Fiber Laser-Based Compact Label-Free Multimodality Nonlinear Optical Microscopy and Its Ex Vivo Bioimaging
December 2024
in “
Microscopy Research and Technique
”
TLDR A new laser-based microscope can clearly image biological structures without labels.
The study introduces a novel multimode nonlinear optical microscopy system utilizing an Er3+-doped femtosecond fiber laser, capable of performing second harmonic generation (SHG), third harmonic generation (THG), and three-photon fluorescence (3PEF) imaging simultaneously. This system successfully visualized various biological structures, including plant cell walls, starch particles, chloroplasts, onion cell linkers, and skin structures such as the stratum corneum, epidermal intercellular structures, hair follicles, and collagen, with spatial resolutions of 1.67 μm for 3PEF, 1.5 μm for THG, and 2.67 μm for SHG. The improved spatial resolution through a high N.A objective allows for diverse biological applications, including in vivo label-free imaging of living animals and deep microscopic imaging.
