Expression and Activity Assay of Human Steroid 5 Alpha-Reductase Type II in CHO Cells

    January 2004 in “ Pharmaceutical biotechnology ”
    Gao Xiao
    TLDR Finasteride effectively inhibits the enzyme steroid 5 alpha-reductase II.
    The study focused on the expression and activity of human steroid 5 alpha-reductase type II (hSRD5A2) in CHO cells. Researchers constructed hSRD5A2 using the pcDNA3 vector and transfected it into CHO cells, creating a cell line that expressed the enzyme. This cell line was used to test the inhibitory effects of selective and non-selective inhibitors on hSRD5A2. The results showed that the enzyme had high activity and was effectively inhibited by the selective inhibitor Finasteride. The study demonstrated a method for screening inhibitors of steroid 5 alpha-reductase II, which was relevant for therapies targeting conditions like benign prostatic hyperplasia.
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