Elicitor Mediated Enhancement of Wedelolactone in Cell Suspension Culture of Eclipta Alba (L.) Hassk
May 2018
in “
Plant Cell, Tissue and Organ Culture (PCTOC)
”
TLDR Adding yeast extract and methyl jasmonate to Eclipta alba cell cultures increased the production of the compound wedelolactone.
In the 2018 study, researchers aimed to optimize the production of wedelolactone, a medicinally valuable compound, through cell suspension culture (CSC) of Eclipta alba. They found that the best biomass accumulation occurred in Murashige and Skoog (MS) medium supplemented with 1.5 mg L−¹ picloram and 0.5 mg L−¹ kinetin at an agitation speed of 120 rpm. The addition of yeast extract, a biotic elicitor, at 150 mg L−¹ increased the wedelolactone content by 1.22-fold after 120 hours of incubation, while 100 µM of methyl jasmonate, an abiotic elicitor, increased wedelolactone content by 1.09-fold without affecting biomass. The study also successfully developed a protocol for regenerating complete plantlets from CSC. These findings have potential applications in the biotechnological production of wedelolactone to meet industrial demands.
