Electrophoretic analysis of human hair keratins

December 1995 in “ Anthropologischer Anzeiger ”

Marcella Folin, Eva Contiero

TLDR Family members have similar hair protein patterns, which could be useful for genetic studies.

The study conducted electrophoretic analysis of non S-carboxymethylated human keratins extracted from the hair of individuals from 3 Italian families. Using SDS-PAGE and isoelectric focusing (IEF), hair fibrous proteins (HFP) were separated into polypeptide bands with molecular weights of 52 KDa, 44 KDa, and 40 KDa, and isoelectric points ranging from pH 7.4 to 4.0. Hair matrix proteins (HMP) were separated by IEF into bands with isoelectric points from pH 7.4 to 5.4, but could not be characterized by SDS-PAGE due to low concentration. Total extracted hair keratins showed additional bands at 16 KDa and 12 KDa. No interindividual variability was observed in electrophoretic patterns, except for one woman possibly affected by cosmetic treatment. Family members exhibited more similar patterns than unrelated individuals, suggesting that electrophoretic analysis of hair keratins could aid in genetic studies.

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research Human hair keratins

90 citations , July 1993 in “Journal of Investigative Dermatology”

research Two-dimensional electrophoretic analysis of human hair keratins, especially hair matrix proteins

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The study conducted a two-dimensional electrophoretic analysis of human hair keratins, focusing on hair matrix proteins (HMP) and hair fibrous proteins (HFP). The proteins were converted to S-carbamoylmethylated derivatives to facilitate separation by isoelectric point and molecular weight. The analysis revealed that SCam-HFP consisted of polypeptides with molecular weights of 41.5–59 kD and isoelectric points (IP) of pH 5.1-6.8. SCam-HMP were divided into acidic and basic groups, with the acidic HMP showing variation among hair samples from 15 individuals in four Japanese families. This method was deemed suitable for biochemical analysis of human hair keratins, particularly nonfilamentous HMP.

Electrophoretic Variability in Human Hair: Comparative Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis of Body and Head Hair Proteins

research Electrophoretic Variability in Human Hair: Comparative Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis of Body and Head Hair Proteins

11 citations , January 1987 in “Electrophoresis”

Keratin proteins are consistent across different hair types from the same person.

Electrophoretic Variability in Human Head Hair: Polyacrylamide Gel Electrophoresis of Hair Proteins in the Presence of Sodium Dodecyl Sulfate and Urea

research Electrophoretic Variability in Human Head Hair: Polyacrylamide Gel Electrophoresis of Hair Proteins in the Presence of Sodium Dodecyl Sulfate and Urea

15 citations , January 1987 in “Electrophoresis”

Human head hair proteins can be typed into eight distinct patterns, useful for genetic and forensic investigations.

Acidic and Basic Hair/Nail (Hard) Keratins: Their Colocalization in Upper Cortical and Cuticle Cells of the Human Hair Follicle and Their Relationship to Soft Keratins

research Acidic and Basic Hair/Nail (Hard) Keratins: Their Colocalization in Upper Cortical and Cuticle Cells of the Human Hair Follicle and Their Relationship to Soft Keratins

356 citations , December 1986 in “The journal of cell biology/The Journal of cell biology”

Hair and nail cells share similar proteins, indicating a common differentiation pathway.

research The complement of native α-keratin polypeptides of hair-forming cells: A subset of eight polypeptides that differ from epithelial cytokeratins

198 citations , October 1986 in “Differentiation”