Development And Validation Of A RP-HPLC Method For The Simultaneous Determination Of Spironolactone And Hydrochlorothiazide In Pure And Pharmaceutical Dosage Form

    January 2023 in “ Zenodo (CERN European Organization for Nuclear Research) ”
    H. Padmalatha
    TLDR A new accurate and reliable method was developed to measure Spironolactone and Hydrochlorothiazide together in medicines.
    A new RP-HPLC method was developed and validated for the simultaneous estimation of Spironolactone and Hydrochlorothiazide in pharmaceutical dosage forms. The method utilized an Inertsil C18 column with a mobile phase of Methanol and Phosphate buffer, achieving retention times of 1.688 mins and 3.282 mins, respectively. The % purity of both compounds was approximately 99.86%, and the method demonstrated high accuracy, precision, and robustness, with a correlation coefficient of 0.999 for both drugs. The method was validated according to ICH guidelines, showing excellent linearity, recovery, and repeatability, making it suitable for routine analysis.
    Discuss this study in the Community →

    Related Community Posts Join

    6 / 483 results

      community Spironolactone and pregnancy planning

      in Female  2 upvotes 6 months ago
      A user with AGA uses topical spironolactone 2% due to side effects from the oral form and is concerned about its use during conception and breastfeeding. They seek alternatives to prevent AGA relapse while planning pregnancy and after childbirth.

      community spironolactone pills to topical

      in Research/Science  6 upvotes 6 months ago
      Crushing spironolactone pills and combining them with minoxidil for topical application may be effective for hair loss, with the addition of finasteride suggested. Adding rosemary and peppermint oils can help mask the unpleasant smell.

      community Does the effect of spironolactone on androgenetic alopecia really weaken over time?

      in Female  4 upvotes 8 months ago
      Spironolactone can remain effective for a long time in treating androgenetic alopecia, but it may not completely stop hair loss. Hair loss treatments like spironolactone, finasteride, and dutasteride slow down hair loss rather than cure it, and their effectiveness can vary based on individual response and the aggressiveness of the condition.

      community Spironolactone VS dutasteride for female pattern hair loss

      in Treatment  7 upvotes 1 year ago
      Spironolactone is more potent and lowers testosterone and DHT, while dutasteride only impacts DHT. For female hair loss, checking hormones and considering treatments like oral minoxidil, spironolactone, or topical minoxidil is recommended.

    Similar Research

    5 / 1000+ results