DermaCult Keratinocyte Expansion Medium: A Serum- and BPE-Free Medium Supporting Long-Term Feeder-Free Expansion of Primary Human Epidermal and Pluripotent Stem Cell-Derived Keratinocytes
November 2022
in “
Journal of Investigative Dermatology
”
DermaCult™ Keratinocyte Expansion Medium serum-free medium bovine pituitary extract-free medium Human Epidermal Keratinocytes HEKs population doublings cell senescence cobblestone morphology basal cell markers KRT10 air liquid interface culture stratified epithelium basal markers suprabasal markers pluripotent stem cell-derived hair-bearing skin organoids DermaCult™ KEM PDs cell aging cobblestone shape layered skin stem cell-derived hair skin organoids
Studysummary This study found that DermaCult™ Keratinocyte Expansion Medium allows for significantly extended growth of human epidermal keratinocytes while maintaining their differentiation potential.
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