Conditioned media from human pluripotent stem cell‐derived cardiomyocytes inhibit the growth and migration of lung cancer cells

The study demonstrated that conditioned media (CM) from human pluripotent stem cell-derived cardiomyocytes (CCM) significantly inhibited the growth, clonogenicity, and migration of lung cancer cell lines A549 and Calu6. In contrast, fibroblast CM (FCM) did not affect the viability of cancer or human foreskin fibroblast cells. An optimized CCM concentration of 30 mg/mL was identified, which impaired cancer cell properties and increased apoptosis rates. Additionally, CCM treatment led to decreased expression of antiapoptotic and stemness genes and increased expression of proapoptotic genes in lung cancer cells. The findings provided evidence for the antitumor potential of cardiomyocyte-derived secretome.