CBL1-CIPK26-Mediated Phosphorylation Enhances Activity of the NADPH Oxidase RBOHC, but Is Dispensable for Root Hair Growth

July 2018 in “ FEBS letters ”

Xinxin Zhang, Philipp Köster, Kathrin Schlücking, Daria Balcerowicz, Kenji Hashimoto, Kazuyuki Kuchitsu, Kris Vissenberg, Jörg Kudla

CBL1 CIPK26 NADPH oxidase RBOHC ROS production Ca2+-dependent phosphorylation S318 S322 root hair growth Arabidopsis RH differentiation Calcium-dependent protein kinase Reactive oxygen species Calcium

Studysummary In this study, researchers identified the CBL1-CIPK26 Ca 2+ sensor-kinase complexes as key modulators of the NADPH oxidase RBOHC crucial for root hair differentiation in plants.

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The study demonstrated that the CBL1-CIPK26 complex phosphorylated and activated the NADPH oxidase RBOHC, enhancing ROS production in a Ca2+-dependent manner, specifically at residues S318 and S322. However, this phosphorylation was not necessary for normal root hair growth in Arabidopsis, as plants expressing a modified RBOHC S318/322A enzyme still showed sufficient ROS production for RH differentiation. This indicated that while CBL1-CIPK26-mediated phosphorylation is critical for RBOHC activity, it is dispensable for RH growth, suggesting alternative regulatory mechanisms in plants.

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