In Vitro Antibacterial and Antioxidant Activities, Pharmacokinetics, and In Silico Molecular Docking Study of Phytochemicals from the Roots of Ziziphus Spina-Christi

The study explored the antibacterial and antioxidant properties of phytochemicals from the roots of Ziziphus spina-christi, traditionally used for treating ailments like hair loss. Five compounds were isolated, with β-sitosteryl-3β-glucopyranoside-6′-O-palmitate showing significant antibacterial activity against E. coli and P. aeruginosa, and strong antioxidant activity. In silico analyses confirmed these findings, highlighting stigmasterol's favorable drug-like properties. The study supports the traditional medicinal use of Z. spina-christi and suggests its compounds have potential therapeutic applications, warranting further research.