Advancing Skin Model Development: A Focus on a Self-Assembled, Induced Pluripotent Stem Cell-Derived, Xeno-Free Approach

This study presents a novel self-assembled skin model (SASM) using hair follicle-derived keratinocytes reprogrammed into induced pluripotent stem cells (iPSC) to create fibroblasts and keratinocytes, forming a xeno-free dermal layer. The model addresses ethical concerns associated with animal testing and the use of bovine collagen in current skin models. The fibroblasts secrete collagen, while the keratinocytes form a functional epidermal layer, both exhibiting metabolic activity and resilience to test substances. The successful development of SASM highlights its potential as an ethical and reliable alternative for dermatological research and regulatory testing.