Induced Pluripotent Stem Cell Line MUSIi006-A Derived from Hair Follicle Keratinocytes as a Non-Invasive Somatic Cell Source

August 2018 in “ Stem cell research ”

Bootsakorn Boonkaew, Lunnathaya Tapeng, Ratchapong Netsrithong, Chinnavuth Vatanashevanopakorn, Kovit Pattanapanyasat, Methichit Wattanapanitch

induced pluripotent stem cells iPSCs hair follicle keratinocytes pluripotent markers embryonic germ layers karyotype mycoplasma contamination viral genome transgenes stem cells hair follicle cells germ layers normal chromosome structure virus-free

TLDR Hair samples can be used to create stem cells easily and non-invasively.

The study derived an induced pluripotent stem cell (iPSC) line, MUSIi006-A, from hair follicle keratinocytes of a 21-year-old Thai woman using Sendai viral vectors. The iPSCs expressed pluripotent markers and could differentiate into three embryonic germ layers. They maintained a normal karyotype, were free from mycoplasma contamination, and had no residual viral genome or transgenes at passage 25. This iPSC line was valuable for developmental biology studies, differentiation protocol development, and as a control for disease-specific iPSCs.

View this study on sciencedirect.com →

Induced Pluripotent Stem Cell Line MUSIi006-A Derived from Hair Follicle Keratinocytes as a Non-Invasive Somatic Cell Source

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research Induced Pluripotent Stem Cell Line MUSIi006-A Derived from Hair Follicle Keratinocytes as a Non-Invasive Somatic Cell Source