Coordination of Force-Generating Actin-Based Modules Stabilizes and Remodels Membranes In Vivo

The study explores how actin-based modules stabilize and remodel membranes in vivo, focusing on secretory granules in mouse salivary glands. It identifies two distinct actin filament populations: linear filaments formed by mDia1 and branched filaments by the Arp2/3 complex. These modules are crucial for membrane integration, with mDia1 providing a stabilizing lattice and the Arp2/3 complex facilitating integration. Experiments on mice and rats show that disrupting these components impairs granule integration, highlighting their essential roles in membrane remodeling. The research provides insights into the cellular mechanisms of membrane dynamics, with implications for secretory processes and potential disease understanding.