Three-Dimensional Imaging and Analysis of the Surface of Hair Fibers Using Scanning Electron Microscopy

July 2007 in “ International Journal of Cosmetic Science ”

C. Tomes, J. T. Jones, C. Carr, Daniel C. Jones

scanning electron microscopy 3D imaging cuticle scale structure atomic force microscopy keratin fibers cuticular scale height SEM AFM

TLDR The new 3D imaging method accurately measures hair surface details quickly.

The study focused on the application of a 3D image analysis package to produce 3D data from 2D scanning electron microscope (SEM) images of hair fibers, specifically analyzing the cuticle scales on the hair's surface. This method allowed for quick and accurate quantification of the scale structure, which was validated through extensive calibration of the SEM and 3D software. The scale heights measured were compared with data obtained using atomic force microscopy (AFM), confirming the accuracy and reproducibility of the technique. Additionally, a 3D-SEM analysis method was developed to monitor individual scales and quantify cuticular scale height after repeated smoothing treatments, establishing a viable method for determining scale height in keratin fibers.

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