Wound-Healing Effects of Frozen-Thawed Allogeneic Fibroblast Sheet Transplantation and Xenogeneic Fibroblast Cell Sheet Transplantation Cultured on a New Substrate

March 2025 in “ Tissue and Cell ”

Yutaka Suto, Koji Ueno, Hiroshi Kurazumi, Wakahiro Kawai, Keita Yoshida, Koji Harada, Tomoko Kondo, Tomoaki Murata, Taro Takami, Kimikazu Hamano

fibroblast sheet transplantation allogeneic fibroblast xenogeneic fibroblast CY-1 substrate hair follicle regeneration VEGF HGF growth factors cryopreservation

TLDR Frozen-thawed fibroblast sheets enhance wound healing and hair growth in mice.

The study explores the wound-healing effects of frozen-thawed allogeneic and xenogeneic fibroblast sheets using a new substrate, CY-1, in mouse models. It finds that these fibroblast sheets maintain their therapeutic efficacy post-cryopreservation, significantly enhancing wound healing and promoting hair follicle regeneration in diabetic and immunodeficient mice. The therapeutic effects are linked to the paracrine action of growth factors like VEGF and HGF, with higher HGF secretion in xenogeneic grafts. The study suggests potential clinical applications for these fibroblast sheets, emphasizing the convenience and cost-effectiveness of using CY-1 substrates, although the mechanisms of fibroblast-induced wound healing remain unclear.

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